Protein_Domain

Part:BBa_K4459002

Designed by: Zi Wang   Group: iGEM22_CUHKSZ   (2022-10-11)


oxygen-insensitive FNR

To make nitrite detection more specific and more accessible, we have to deal with two more things. We notice that FNR is also a transcriptional factor of the nrfA gene. In fact, FNR is acting as a switch for many bacteria from aerobic to anaerobic metabolism. By direct interaction with oxygen, the Fe-S complex of FNR is quickly converted to [3Fe-4S]+ or a [2Fe-2S]2+, inactivating FNR. Thus one obvious limitation of our project is that we have to ensure an anabolic environment, which may increase the failure or bias of the detection. To solve this we try to introduce a modified oxygen-insensitive FNR mutant protein by substituting either Cys-23 or Cys-122 was substituted for Ser and substitution at position 154.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 568
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 568
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 568
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 568
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 568
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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